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. 2020 Apr 15;10:6441. doi: 10.1038/s41598-020-63418-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Vectashield (VS) on the fluorescence of AF647- and AF488-labelled microtubules. (a) Widefield images of ND7/23 cells labelled with AF647-conjugated anti-tubulin β3 (AF647-TUBB3) antibody in PBS (upper panels) and in VS (lower panels). To ensure the quenched AF647 signal in VS is visible, the same images are shown on the right with different linearly adjusted brightness and contrast levels (as indicated by look-up table (LUT) intensity scale bars). (b) Widefield images of ND7/23 cells labelled with AF488-conjugated anti-tubulin β3 (AF488-TUBB3) antibody in PBS (upper panel) and in VS (lower panel). Brightness and contrast were linearly adjusted to show the same display range in both PBS and VS conditions (as indicated by LUT intensity scale bars).