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. 2020 Apr 15;10:6441. doi: 10.1038/s41598-020-63418-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of dSTORM parameters of AF647 (magenta) and AF(+)647 (blue) in different imaging media for (a) tubulin β3, (b) βII spectrin and (c) neurofilament light chain (NfL). The box plots show photon counts, lateral localisation precision, FRC and molecular density in GLOX BME, 25% Vectashield (25% VS) and Vectashield (VS). For each of the parameters, 10 images per condition were analysed. Circles indicate outliers. * indicates significance with p < 0.05, obtained by using separate analyses of variance (ANOVAs) with post-hoc Bonferroni comparisons. ** indicates significance with p < 0.004167, obtained using 12 t-tests with Bonferroni corrections. Detailed explanations on statistical analysis can be found in the Supplementary Information.