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. 2020 Apr 15;11:1846. doi: 10.1038/s41467-020-15603-3

Fig. 1. The KIX-PPD complex acts maternally to control seed development.

Fig. 1

a, b The seeds (a) and 8-day-old seedlings (b) of Col-0, kix8-1, kix9-1, kix8-1 kix9-1, ppd1-2, ppd2-1, ppd1-2 ppd2-cr, ppd1-cr ppd2-1, and kix8-1 kix9-1 ppd1-2 ppd2-cr plants. c–e The relative seed area (c, n = 100), 100 seed weight (d, n = 10), and cotyledon area (e, n = 30) of Col-0, kix8-1, kix9-1, kix8-1 kix9-1, ppd1-2, ppd2-1, ppd1-2 ppd2-cr, ppd1-cr ppd2-1, and kix8-1 kix9-1 ppd1-2 ppd2-cr. Seeds from the third to seventh silique on the stem of six plants were used for analysis. Cotyledons from the 8-day-old seedlings were used for analysis. f, g The relative area of F1 seeds (f) and F2 seeds (g) from Col-0/Col-0 (C/C), Col-0/kix8-1 kix9-1 ppd1-2 ppd2-cr (C/kkpp), kix8-1 kix9-1 ppd1-2 ppd2-cr/Col-0 (kkpp/C), and kix8-1 kix9-1 ppd1-2 ppd2-cr/kix8-1 kix9-1 ppd1-2 ppd2-cr (kkpp/kkpp) plants (n = 100). h Ovules of Col-0 and kix8-1 kix9-1 ppd1-2 ppd2-cr plants at 0 DAP (days after pollination). i–l The seed area (i), outer integument length (j), outer integument cell number (k), and outer integument cell length (l) of Col-0 and kix8-1 kix9-1 ppd1-2 ppd2-cr plants at 0, 2, 4, and 6 DAP (n = 33). Ovules and seeds from six siliques, which were from the fourth silique on the stem of six plants, were used for analysis. Scale bars, 0.5 mm (a), 0.2 cm (b), and 50 μm (h). Error bars represent ±SE. Different lowercase letters above the columns indicate the significant difference among different groups, one-way ANOVA P-values: P < 0.05. * indicates significant difference from the Col-0, one-way ANOVA P-values: *P < 0.05 and **P < 0.01.