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. 2020 Apr 15;11:1846. doi: 10.1038/s41467-020-15603-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Split luciferase complementation assays showing the interactions between PPD1/2 and MYC3/4. MYC3/4-nLUC and cLUC-PPD1/2 were coexpressed in N. benthamiana leaves. The luciferase activity was detected at 2 days later after infiltration. b The FRET-FLIM assays showing that MYC3/4 interact with PPD1/2 in N. benthamiana leaves. CFP fluorescence lifetime was obtained at 2 days later after coinfiltrating with different combinations of 35S:MYC3-CFP, 35S:MYC4-CFP, 35S:PPD1-YFP, 35S:PPD2-YFP, and 35S:DEL1-YFP constructs. 35S:DEL1-YFP was used as a negative control. c Pull-down analyses showing the interactions between PPD1/2 and MYC3/4 in vitro. GST-MYC3 and GST-MYC4 were incubated with MBP-PPD1, MBP-PPD2, and MBP, respectively. Proteins were pulled down by MBP-Trap-A agarose beads and detected by Western blot with anti-GST or anti-MBP antibody. d Co-immunoprecipitation analyses showing the interactions between PPD1/2 and MYC3/4 in Arabidopsis. Total protein extracts of 35S:Myc-PPD1/2;35S:GFP and 35S:Myc-PPD1/2;35S:GFP-MYC3/4 plants were incubated with GFP-Trap agarose beads. Precipitates were detected by Western blot with anti-GFP or anti-Myc antibody. e Pull-down analyses showing that PPD1/2 are required for the interactions between KIX8 and MYC3/4. His-KIX8 was incubated with GST-MYC3 or GST-MYC4 and MBP-PPD1 or MBP-PPD2. Proteins were pulled down by the Ni-NTA agarose beads and detected by Western blot with anti-GST, anti-MBP, or anti-His antibody. f Co-immunoprecipitation analyses showing that KIX8/9 and MYC3/4 are in a protein complex in Arabidopsis. Total protein extracts of 35S:Myc-KIX8/9;35S:GFP and 35S:Myc-KIX8/9;35S:GFP-MYC3/4 plants were incubated with GFP-Trap agarose beads. Precipitates were detected by Western blot with anti-GFP or anti-Myc antibody.