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. 2020 Apr 15;11:1846. doi: 10.1038/s41467-020-15603-3

Fig. 7. GIF1 acts genetically with the KIX-PPD-MYC complex to control seed size.

Fig. 7

a, b The seeds (a) and 8-day-old seedlings (b) of Col-0, gif1, myc3 myc4, gif1 myc3 myc4, kix8-1 kix9-1, gif1 kix8-1 kix9-1, ppd1-2 ppd2-cr, gif1 ppd1-2 ppd2-cr, 35S:SAP, and 35S:SAP;gif1 plants. c–e The relative seed area (c, n = 100), 100 seed weight (d, n = 10), and cotyledon area (e, n = 30) of Col-0, gif1, myc3 myc4, gif1 myc3 myc4, kix8-1 kix9-1, gif1 kix8-1 kix9-1, ppd1-2 ppd2-cr, gif1 ppd1-2 ppd2-cr, 35S:SAP, and 35S:SAP;gif1 plants. f The TPL-KIX-PPD-MYC complex associates with the G-box sequence of GIF1 promoter and represses its expression. g The transcriptional repression of GIF1 is relieved by SAP modulating the KIX-PPD module for 26S proteasome degradation. Without the KIX-PPD complex, the binding ability of MYC3/4 with the promoter of GIF1 is decreased and the GIF1 expression is increased. Seeds from the third to seventh silique on the stem of six plants were used for analysis. Cotyledons from the 8-day-old seedlings were used for analysis. Scale bars, 0.5 mm (a) and 0.2 cm (b). Error bars represent ±SE. Different lowercase letters above the columns indicate the significant difference among different groups, one-way ANOVA P-values: P < 0.05.