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. 2020 Apr 9;11:633. doi: 10.3389/fmicb.2020.00633

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Copyright © 2020 Zhou, Peng, Liu, Chang, Xiao, Liu and Fang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of GspInv in K. pastoris. (A) Time course of invertase activity, protein content, and K. pastoris concentration (OD₆₀₀) in the extracellular medium of K. pastoris cultures producing recombinant GspInv. The data presented are the average values from triplicate cultures (repeats of experiments gave similar results). (B) Coomassie brilliant blue R250 stained 15% SDS-PAGE gel of GspInv in crude culture supernatant (lane 1) and after ion-exchange chromatography (lane 2). (C) Zymogram gel of GspInv in crude culture supernatant.