FIGURE 3.

Effects of pH and temperature on the activity and stability of GspInv, CBM₂₄DG-GspInv, and cellulose-CBM₂₄DG-GspInv. (A) pH optimum. Samples were incubated at 45°C. (B) Temperature optimum. Samples were incubated at pH 5.0. (C,D) Thermostability at 45°C and 40°C, respectively. Samples were incubated at pH 5.0. (E,F) pH stability at pH 5.5 and pH 6.0, respectively. Samples were incubated at 4°C. Standard deviations and values were calculated from triplicate technical repeats of measurements.