FIGURE 1.

Schematic diagram of tube-in-tube devices for cell-free protein synthesis and fluorescence gained from devices. (A) Cell-free protein synthesis (CFPS) system was injected into the inner tube of the tube-in-tube reactor, surrounded by the controllable gas. (B) Normalized fluorescence of sfGFP synthesized in cell-free systems in the tube-in-tube reactor oxygen tensions of 0, 10, 21, 30, 50, 70, and 100% in 2 h and 37°C. (C) Normalized fluorescence of sfGFP synthesized in cell-free systems in the tube-in-tube reactor with oxygen tensions of 0, 2, 4, 6, 8, and 10% in 2 h and 37°C. Values were calculated as the mean of biological replicates.