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. 2020 Apr 15;40(4):BSR20200259. doi: 10.1042/BSR20200259

Figure 2. Effect of tanshinone II A on the regulation of Akt/PI3K and NF-kB/LC3 signaling on HeLa cells.

Figure 2

(A–C) Representative Western blot analysis (A) and quantification (B,C) of Akt, p-Akt and PI3K protein on HeLa cells at different concentrations (0, 2, 4, 8 μm) after treatment for 72 h. Protein expression was normalized to β-actin levels. *P<0.05, **P<0.01, ***P<0.005, ****P<0.001. (D,E) qPCR analyses of the relative mRNA levels of LC3 and NF-kB on HeLa cells after treatment for 72 h. Gene expression was normalized to β-actin mRNA level. *P<0.05, **P<0.01, ***P<0.005. (F–H) Representative Western blot analysis (F) and quantification (G,H) of LC3-I and NF-kB p65 protein on HeLa cells after treatment for 72 h. *P<0.05, **P<0.01, ***P<0.005, ****P<0.001.