Table 1.

Primers and annealing temperature for relative real-time RT-PCR and semi-quantitative RT-PCR.

Genes	Primer sequence (5'-3')	Annealing (°C)
Insulin	F:ATTGTTCCAACATGGCCCTGT	61
	R:TTGCAGTAGTTCTCCAGTT
Glucagon	F:ACGAATACATTTCCCTTTAGCG	60
	R:TGTTGTTGTAATCCAGGTGTCG
GLUT4	F:CTCTCAGGCATCAATGCTGTT	60
	R:GAGACCAACGTGAAGACGGTA
Insulin receptor	F:CTGGAGAACTGCTCGGTCATT	60
	R:GGCCATAGACACGGAAAAGAAG
IL-1β	F:TGAGGCTGACAGACCCCAAAAGAT	60
	R:GCTCCACGGGCAAGACATAGGTAG
TNF-α	F:CACGTCGTAGCAAACCACCAA	60
	R:GTTGGTTGTCTTTGAGATCCAT
β-actin	F:ACTTCGAGCAGGAGATGGCC	60
	R:CCCAAGAAGGAAGGCTGGAA
GPR147	F:AGCCTCACCTTCTCCTCCTACTACC	60
	R:AGTGATAAGGTTGTCCACAAGGGTT
RFRP-3	F:GAGGAATCCCAAAAGGGGTAAAGG	60
	R:GTGATGCGTCTGGCTGTTGTTCT
GAPDH	F:ACCACAGTCCATGCCATCAC	55
	R:TCCACCACCCTGTTGCTGTA