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. 2020 Apr 9;11:619. doi: 10.3389/fimmu.2020.00619

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Copyright © 2020 Tatomir, Rao, Boodhoo, Vlaicu, Beltrand, Anselmo, Rus and Rus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of MBP, PLP, and cyclin D1 increases during OLG differentiation in vitro. OPCs purified from neonatal rat pups were cultured in an OLG differentiation medium, and expression of MBP and PLP mRNAs (A,B) was analyzed by real-time PCR. The expression of the mRNA at the beginning of the experiment (0 h) was considered to be 100, and the results are shown as -fold change. In addition, MBP (C) and cyclin D1 (D) protein expression was analyzed by Western blotting at the indicated time periods. The protein is expressed as a ratio to β-actin, and a representative Western blot is shown in (E). Significantly higher levels of MBP and PLP associated with an increase of cyclin D1 in the first 24 h were found during OLG differentiation. The results are expressed as mean ± SEM (n = 3). *p < 0.05, **p < 0.01.