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. 2020 Apr 9;11:619. doi: 10.3389/fimmu.2020.00619

FIGURE 4.

FIGURE 4

Induction of SIRT1, p-SIRT1 and cyclin D1 expression by sublytic C5b-9 in OLGs. OPCs were differentiated for 72 h, and OLGs were then exposed to sublytic levels of C5b-9 derived from either NHS or assembled purified complement components for the indicated periods of time. Expression of SIRT1 mRNA (A), SIRT1 protein (B), phospho-SIRT1 (Ser27) (D), and cyclin D1 protein (E) was examined as described in section “Materials and Methods.” The expression of the mRNA at the beginning of the experiment (CTR) was considered to be 100, and the results are shown as -fold change (A). The expression of SIRT1, phospho-SIRT1 and cyclin D1 proteins was normalized to β-actin, and representative blots are shown in (C) and (F). We found that sublytic C5b-9 induced SIRT1 expression and activation, along with increased cyclin D1 expression. Results are expressed as mean ± SEM (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001.