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. 2020 Apr 9;11:619. doi: 10.3389/fimmu.2020.00619

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Copyright © 2020 Tatomir, Rao, Boodhoo, Vlaicu, Beltrand, Anselmo, Rus and Rus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of sublytic C5b-9 on progenitors and mature OLG markers. OLGs were stimulated with sublytic C5b-9 for the indicated periods of time and analyzed for the expression of MBP mRNA (A), PLP mRNA (B), and MBP protein (C). OLGs were also stimulated with sublytic C5b-9 for 3 h and analyzed for the mRNA expression of NG2/CSPG4 (D) and Sox10 (E). The expression of the mRNA at the beginning of the experiment (CTR) was considered to be 100, and the results are shown as -fold change (A,B,D,E). MBP protein expression was normalized to β-actin, and a representative blot is shown in (C). We observed a downregulation of the mature markers MBP, PLP, and SOX10 and a re-expression of NG2/CSPG4, suggesting the dedifferentiation of OLGs to an OPC-like phenotype after exposure to sublytic C5b-9. Data are expressed as mean ± SEM (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.