FIGURE 8.

Effect of SIRT1 and c-jun inhibition on C5b-9-induced [³H] thymidine incorporation. OLGs were incubated with C5b-9 assembled from purified complement components, either alone or after a 4-h preincubation with a c-jun antisense oligonucleotide (20 μM), a c-jun sense oligonucleotide (20 μM), or sirtinol (200 μM), a SIRT1 inhibitor. The cells were then incubated with [³H] thymidine and processed, and radioactivity was detected by liquid scintillation as counts per minute (CPM). Our data show that S-phase entry induced by sublytic C5b-9 in OLGs is c-jun and SIRT1-dependent. Data are expressed as mean ± SEM (n = 3). ****p < 0.0001.