TABLE 2.
Antibodies used for western blotting.
| Primary antibodies | |||||
| Antigen | Species | Clone | Dilution | Source | Catalog no. |
| Phospho-SIRT1 (Ser27) | Rabbit | Polyclonal | 1:400 | Biorbyt, Cambridge, United Kingdom | orb6958 |
| SIRT1 | Rabbit | D1D7 | 1:1000 | Cell Signaling Technology, Danvers, MA, United States | 9475 |
| Cyclin D1 | Rabbit | DCS6 | 1:1000 | Cell Signaling Technology | 2926 |
| H3K9me3 | Mouse | 2AG-6F12-H4 | 1:5000 | Active Motif, Carlsbad, CA, United States | 39286 |
| MBP | Rabbit | Polyclonal | 1:1000 | GenScript, Piscataway, NJ, United States | A01407 |
| β-actin | Mouse | 2D4H5 | 1:35000 | Proteintech, Rosemont, IL, United States | 66009-1-Ig |
| β-tubulin | Mouse | 1D4A4 | 1:30000 | Proteintech | 66240-1-Ig |