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. 2020 Apr 1;4(7):1270–1283. doi: 10.1182/bloodadvances.2019001323

Figure 4.

Figure 4.

C587R mutant peripheral lymphocytes are sensitized to apoptosis and underrepresented under competitive condition (A) Peripheral lymphocytes carrying the C587R mutation are sensitized to apoptosis. Growth factor deprivation assay of FACS-sorted naive CD4, CD8 T cells and Fo B cells from either C587R/+ or WT animals. Representative experiment of 3. Each point is the average of 3 technical replicates and bar represents standard deviation. (B) The deleterious effect of the C587R mutation on B-cell survival is masked by the deletion of Bax and Bak1. Representative experiment of 2. Each point is the average of 3 technical replicates and bar represents standard deviation. (C) BM competitive transplant assay. (D) Peripheral B- and T-cell compartments are underrepresented in competitive BM chimera. Twelve weeks posttransplant, the relative representation of “test” and “competitor” cells among splenic lymphocytes was assessed. Data show mean with SD. **P < .01; ***P < .001; and ****P < .0001.