Figure 7.

The C587R mutation sensitizes lymphocytes to translation inhibition. (A) FACS-sorted lymphocytes were submitted to various concentrations of LMB (A) or CHX (B) for 24 hours. Representative experiment of 2. Each point is the average of 3 technical replicates and bar represents standard deviation. (C) Mixed BM chimeras were set up as shown for Figure 4C. Representative FACS plot showing chimerism in the B-cell compartment: WT CD45.1⁺ “competitor cells” together with either WT (top panel) or C587R/+ mutant CD45.2⁺ “test cells” (bottom panel). (D) Increased sensitivity to translation inhibition is intrinsic to C587R-mutant Fo B cells. Representative experiment of 2. Each point is the average of 3 technical replicates and bar represents standard deviation. (E) Loss of Bax and Bak1 in B cells masks the deleterious effect of the C587R mutation in response to translation inhibition. Representative experiment of 2. Each point is the average of 3 technical replicates and bar represents standard deviation.