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. 2020 Apr 9;4(7):1388–1406. doi: 10.1182/bloodadvances.2019000699

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Figure 2. — Among CD56^dim CD16⁺ NK cells is a distinct subset of CD57⁺ NK cells. (A) Expression distribution of each cluster (violin plots) using unstimulated cells only, looking at canonical human NK cell markers, grouped by cytotoxicity, inhibitory and activating receptors, cytokines and chemokines, cytokine and chemokine receptors, and adhesion molecules. The shape represents the distribution of cells based on their log(+1) expression values. The color scale represents the mean expression. (B) Percentage of individual NK cells expressing 1, 2, 3, or 4 different KIRs across each cluster, calculated using the full data set of 8462 cells. (C) Comparison of average gene expression values for cluster 0 and cluster 1 in unstimulated cells only. Genes with fold change >0.4 are highlighted. (D) Comparison of average gene expression values for clusters 0 and 1 between unstimulated and IL-2–stimulated cells. Genes with a fold change >0.5 and Bonferroni-corrected P < .05 are highlighted. (E) Selected GO terms using all conserved markers upregulated or downregulated within the individual clusters with an adjusted P < .05. (F) Dot plot (left) of selected markers of interest within unstimulated cells only across the different clusters. The size of the dot represents the percentage of cells expressing the markers, and the color encodes the average scaled expression values. Heat map (right) of the same markers of interest within unstimulated cells only. Clusters are plotted in columns, and genes are shown in rows. Gene expression is color coded using average scaled expression values per cluster, based on a z-score distribution, ranging from low expression (purple) to high expression (yellow). (G) Comparison of average gene expression values for cluster 2 between unstimulated and IL-2–stimulated cells. Genes with a fold change >0.5 and Bonferroni-corrected P < .05 are highlighted. (H) Selected GO terms using all conserved markers upregulated or downregulated within this cluster with an adjusted P < .05.

Figure 2. — Among CD56^dim CD16⁺ NK cells is a distinct subset of CD57⁺ NK cells. (A) Expression distribution of each cluster (violin plots) using unstimulated cells only, looking at canonical human NK cell markers, grouped by cytotoxicity, inhibitory and activating receptors, cytokines and chemokines, cytokine and chemokine receptors, and adhesion molecules. The shape represents the distribution of cells based on their log(+1) expression values. The color scale represents the mean expression. (B) Percentage of individual NK cells expressing 1, 2, 3, or 4 different KIRs across each cluster, calculated using the full data set of 8462 cells. (C) Comparison of average gene expression values for cluster 0 and cluster 1 in unstimulated cells only. Genes with fold change >0.4 are highlighted. (D) Comparison of average gene expression values for clusters 0 and 1 between unstimulated and IL-2–stimulated cells. Genes with a fold change >0.5 and Bonferroni-corrected P < .05 are highlighted. (E) Selected GO terms using all conserved markers upregulated or downregulated within the individual clusters with an adjusted P < .05. (F) Dot plot (left) of selected markers of interest within unstimulated cells only across the different clusters. The size of the dot represents the percentage of cells expressing the markers, and the color encodes the average scaled expression values. Heat map (right) of the same markers of interest within unstimulated cells only. Clusters are plotted in columns, and genes are shown in rows. Gene expression is color coded using average scaled expression values per cluster, based on a z-score distribution, ranging from low expression (purple) to high expression (yellow). (G) Comparison of average gene expression values for cluster 2 between unstimulated and IL-2–stimulated cells. Genes with a fold change >0.5 and Bonferroni-corrected P < .05 are highlighted. (H) Selected GO terms using all conserved markers upregulated or downregulated within this cluster with an adjusted P < .05.