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Internalization of DCR-2. (A) Internalization kinetics of DCR-2 on HL-60 (% internalized = relative MFI of total − relative MFI of surface staining). (B) Immunofluorescent microscopy of HL-60, CD14+ monocytes, and primary AML at 0 minutes (no internalization) and 30 minutes showing internalization of CD300f detected by LMIR-3 antibody. Isotype control shown is at 30 minutes. Images were taken at 63× magnification using a 63× HC PL APO CS2 NA 1.40 lens.
