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. 2020 Apr 9;11:541. doi: 10.3389/fmicb.2020.00541

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Copyright © 2020 Hetman, Mutschall, Carrillo, Thomas, Gannon, Inglis and Taboada.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of growth dynamics of a mixed culture under enrichment and non-enrichment conditions via digital droplet PCR (ddPCR). Strain mixture “C” was grown in enrichment and non-enrichment broth with DNA extracted at 4-h intervals over a 24-h incubation period. For each time point, strain specific-DNA was quantified using ddPCR to estimate mean relative abundance of each strain in the mixture. After 4 h in non-enrichment recovery, moderate differences were observed between the growth rates of the four strains, with final relative abundances ranging from 13.3 to 35.3% after 24 h. The mean of three replicates is shown. Filled circles denote time points in which there is significant difference (p < 0.05) in relative abundance between enrichment and non-enrichment recovery methods.