TABLE 2.

Sequences and modifications of primers and probes designed for digital droplet PCR assay.

Strain	Target	Product	Sequence	Modification
CE_R_11_0249	cj0033-F	Primer	TGGGATAAAAGGGGTGAGAA
	cj0033-R	Primer	CGTGAAGCCAAGTAAAACCAA
	cj0033-Hyb	Probe	TGTTTCGAGAATTCGGGATTTTATGG	FAM
All (Control)	Cj0102-F	Primer	CAAAGCACAAAAAGTGAGATTT
	Cj0102-R	Primer	CAACATTGTGAATAAGCTCCAT
	Cj0102-Hyb	Probe	TGCTCCTTATGCAAAAGGTGGT	HEX
CI_4820	cj0569-F	Primer	TTGGTTTGGACATTTAGCATC
	cj0569-R	Primer	GCTAGTGTTTGTCTATGTTGTC
	cj0569-Hyb	Probe	TGATTGGTGTGGATCTAGTGGAGG	FAM
CE_R_11_0073	cj1431c-F	Primer	AATTGCAGGAAGGGATGATG
	cj1431c-R	Primer	CAAATTTGCCCAAGGAATCA
	cj1431c-Hyb	Probe	TGGTTTTAAATTCGGTTTTGTATGGAGA	FAM
07_1875	cj1550c-F	Primer	GGAAAGATGGTTGAATGGAAAG
	cj1550c-R	Primer	TCTAAGGCTAACAAAGCATCG
	cj1550c-Hyb	Probe	AGCAAGTAATGTGAATATGCCTAGCGT	FAM