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The effects of st-Ht31 on contraction of human airway smooth muscle strips. Isolated human tracheal smooth muscle strips were incubated without or with st-Ht31 (50 μM). (A) Methacholine-induced isometric contraction was measured. N = 7–8. ***p < 0.001 compared to basal as determined by an extra sum-of-squares F-test. (B,C) The expression of α-sm-actin (B) and PCNA (C) was determined in the tracheal strips by immunoblotting. N = 4–5, *p < 0.05 compared to basal.
