Figure 3.

Jun Disturbs the Regular Architecture of the Growth Plate

Further statistical analyses and raw data are listed in the Data S3. Two-sided t tests were used to determine statistical significances between −JUN and +JUN. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

(A) Representative CT images of growth plates (Ai). CT-based measurements of bone lengths and growth plate lengths in all long bones, normalized to the values without Jun (Aii). Each of four animals contributed multiple long bones to the analysis (n = 13–22 for bone lengths, n = 6–10 for growth plates). Each data point represents individual bone measurements from two independent experiments.

(B) Representative pentachrome stainings of growth plates (Bi) indicating the reserve zone (R), proliferative zone (P), and hypertrophic zone (H). Scale bar,100 μm. Lengths of the different zones without and with Jun induction (n = 14–20) (Bii). Each data point represents an individual bone measurement. Each mouse contributed each type of bone. Representative osseous outgrowth (encircled area) under JUN induction (Biii). Scale bar, 100 μm.

(C) Representative EdU stainings without and with Jun induction (Ci). Scale bar, 100 μm. Counting of EdU⁺ cells per 100 μm growth plate width (n = 9–15) (Cii). Each data point represents an individual bone measurement. Each mouse contributed each type of bone.

(D) Representative immunostaining against Jun of the growth plate and corresponding percentage of Jun⁺ cells in the growth plate, showing that all zones of the growth plate express. Scale bar, 100 μm.

(E) Representative immunostaining against Jun of the mature bone and corresponding percentage of Jun⁺ cells in the mature bone (n = 4–6). Data points represent individual measurements from two independent experiments. Scale bar, 100 μm.

Ns, not significant.