Figure 1.

Loss of Dnmt3a and Tet2 Enhance Self-Renewal in HSCs to Different Degrees

(A) HSC serial transplantation schematic. In descending column order—contribution of 200 Control^Mx1, Dnmt3a-KO^Mx1 (3aKO), and Tet2-KO^Mx1 (T2KO) HSCs to peripheral blood, lineage chimerism, HSC frequency, and HSC number in (B) primary (CNT n = 28; 3aKO n = 24; T2KO n = 22), (C) secondary (CNT n = 27; 3aKO n = 19; T2KO n = 21), and (D) tertiary (CNT n = 33; 3aKO n = 23; T2KO n = 19) transplants. (E) Self-renewal and (F) differentiation quotients of indicated HSC genotypes after each transplant. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.001. Mean ± SEM is shown.