Figure 4.

Dnmt3a and Tet2 Loss of Function Alter Hematopoietic Progenitor Function through Distinct Molecular Mechanisms

(A) Principal-component analysis plot of gene expression in Control^Vav HSCs (n = 3) and MPP3 (n = 4), Dnmt3a-KO^Vav HSCs (n = 3), and Tet2-KO^Vav HSCs (n = 4) and MPP3 (n = 4).

(B) Venn diagrams displaying DEG overlap in Dnmt3a-KO^Vav and Tet2-KO^Vav HSCs compared with Control^Vav HSCs.

(C) Heatmaps displaying DEGs (p < 0.05, fold-change >1 or <−1) between Control^Vav and Tet2-KO^Vav HSCs and MPP3.

(D) Gene set enrichment analysis showing differentially regulated pathways between Control^Vav and Tet2-KO^Vav MPP3.

(E) Gene score enrichment plot of “Hallmark TNFα Signaling via NFkB” gene set in Tet2-KO^Vav MPP3.

(F) ATAC-seq heatmaps from Control^Vav, Dnmt3a-KO^Vav, and Tet2-KO^Vav mice. Signals displayed are peaks 1 kb up- and downstream of transcription start sites of protein coding genes.

(G) Multi-dimensional scaling plot with distances approximating the largest log₂ fold-changes in the top 500 peaks between ATAC-seq samples.