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. 2020 Mar 26;14(4):703–716. doi: 10.1016/j.stemcr.2020.02.010

Figure 2.

Figure 2

HIV-Infected iMicroglia Produce an Inflammatory Response, and Respond to EFZ

(A) Immunostaining showing reduced percentage of P24+ (red) IBA1+ (green) iMg in HIV-infected mono-cultures + EFZ treatment compared with infected cultures with no EFZ treatment. Scale bar represents 50 μm.

(B) Percentage of P24+ cells in mono-culture iMg at D15 for infected and Inf + EFZ conditions. n = 4 infections from 3 cell lines, error bars represent SEM.

(C) Percent of P24+ single nucleated and multinucleated iMg in mono-culture for infected and Inf + EFZ conditions. n = 4 infections from 3 cell lines.

(D) Percent of P24(−) single nucleated and multinucleated iMg in mono-culture for Inf and Inf + EFZ conditions. n = 4 infections from 3 cell lines.

(E) Reverse transcriptase activity of Uninf, Inf, and Inf + EFZ (20 nM) iMg show productive infection and response to EFZ. n = 3 independent infections of WT6, one-way ANOVA, Dunnett's post hoc analysis; ∗p < 0.05, ∗∗∗∗p < 0.0001; error bars represent SEM.

(F–I) Cytokine analysis of infected iMg mono-culture displays increase in IL-1b (F), IL-8 (G), TNF-α (H), and IL-1a (I) production in the infected iMg. n = 3 independent infections of WT6, one-way ANOVA, Dunnett's post hoc analysis; ∗p < 0.05, ∗∗p < 0.01; error bars represent SEM.