Figure 5.

Methylation of CpG island. The percentage of methylated CpG island in the eyestalk ganglion of females at early-vitellogenic stage compared with the eyestalk ganglion at late-vitellogenic stage (A), the muscle of females (C), and the eyestalk ganglion of males (E). The average methylation of CpG island between the eyestalk ganglion of females at early-vitellogenic stage and the eyestalk ganglion at late-vitellogenic stage (B), the muscle of females (D), the eyestalk ganglion of males (F). Different letters represent significant difference between groups (p < 0.05, one-way ANOVA followed by the Tukey's post hoc test). Each bar represents the mean ± SEM (n = 3).