FIGURE 1.
Casp-1/11 but not RIPK3 is necessary for the host ability to clear LM-OVA infection. All the experimental mice were infected for 103 CFU of LM-OVA for 3 and 7 days, respectively. Spleen size was measured at day 3 and day 7 post-infection. Spleen from each infected mice was harvested and processed individually to make single-cell suspensions. CFU/spleen was determined by plating 10-fold serial dilutions of single-cell suspension from each individual mouse on BHI-Streptomycin agar plates. (A,C) Representative images of the spleen size and (B,D) bacterial burden were measured in infected mice at 3 (A,B) and 7 (C,D) days after LM-OVA infection. Results expressed as the mean of five individual mice per group and are representative of three independent experiments. Statistical analysis was performed by using two-way ANOVA followed by Bonferroni post-tests. ***p < 0.001.