FIGURE 5.

RIPK3 and/or Casp-1/11 deficiencies reduce antigen-specific CD8⁺ T cells degranulation and intracellular cytokine production in response to LM-OVA. (A) Representative images of frequency of LAMP-1-positive OVA-specific CD8⁺ T cells for LM-OVA-infected and non-infected (control) groups and the (B) frequency variation of LAMP-1⁺-CD8⁺ T cells observed on the LM-OVA-infected group. Immediately, representative image of intracellular H2-K^b-SIINFEKL-specific (C) IFN-γ and (E) TNF-α-producing CD8⁺ T cell expression on control and infected groups. The frequency of these cell populations, H2-K^b-SIINFEKL-specific (D) IFN-γ and (F) TNF-α-producing CD8⁺ T cells, after 7 days of infection with LM-OVA was schematized. Frequencies described in (B,D,F) bar graphs were representative of experiments done in triplicate, and the data depict mean ± SEM of five individual mice per group. Statistical analysis was performed by using two-way ANOVA followed by Bonferroni post-tests. ***p < 0.001.