TABLE 1.
Strains and plasmids used in this study.
| Strains and plasmids | Previous ID/description | Serotype | References | Nisin MIC (mg/mL)1 |
| Strains L. monocytogenes | ||||
| FSL X1-0001 | 10403S | 1/2a | Bishop and Hinrichs, 1987 | 0.016 |
| FSL R9-5621 | SAMN02566964 | 1/2a | CDC, 2012 | 0.016 |
| FSL R9-5623 | SAMN02265450 | 4b | CDC, 2013 | 0.016 |
| FSL R9-5624 | SAMN02689015 | 1/2b | CDC, 2014 | 0.031 |
| FSL R9-5625 | SAMN02950474 | 4b | CDC, 2015 | 0.031 |
| FSL D4-0041 | 10403S ΔdltA | 1/2a | Tokman et al., 2016 | 0.008 |
| FSL B2-0451 | 10403S ΔmprF | 1/2a | This study | 0.004 |
| FSL B2-0445 | 10403S ΔdltAmprF | 1/2a | This study | 0.004 |
| P. cerevisiae | ||||
| FSL C8-0053 | P. cerevisiae E66 | N/A | Gift from Randy Worobo (Cornell University) | 0.004 |
| Plasmids | ||||
| pBMB100 | ΔmprF on pKSV7 | N/A | This study | |
| pKSV7 | Integrative shuttle vector | N/A | (Smith and Youngman, 1992) |
1Minimal inhibitory concentration (MIC) from triplicate plates. MIC reported as the lowest concentration of nisin that inhibited visible growth (Wiegand et al., 2008).