Figure 1.

Growth of human metastatic cancer cells with ammonium. Cancer HeLa, MDA-MB-231, PC3, and HCT116 cells (20 × 10³ cells/well) were grown in 96-well plates in the presence of 0 (■), 0.5 (•), 1 (▴), 5 (♦), and 10 (▾) mM NH₄Cl. Cell growth was monitored by counting cellular density every 24 h. Viability was >90% in all culture conditions. Data shown represent the mean ± SD of at least three different preparations. Statistical analysis was performed using one-way ANOVA with Scheffé comparison test. *P < 0.01 vs. 1, 5, or 10 mM NH₄Cl.