Figure 5.

Kinetics of the hepatoma mitochondria reverse GDH reaction. For these representative experiments, 219 μg protein of HepM was incubated in KME buffer at pH 7.20 and 37°C. The saturation curves with variables NH₄Cl and ADP were fitted to the Hill equation, whereas the saturation curves with variables 2-OG and NADPH were fitted to the Michaelis–Menten equation. The units of the GDH activity were Δabsorbance at 340 nm min⁻¹.