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. 2020 Apr 9;10:429. doi: 10.3389/fonc.2020.00429

Figure 6.

Figure 6

Glutamate dehydrogenase kinetics obeys the ligand exclusive binding MWC model. For these representative experiments, 280 μg protein of HepM (A), and 90 μg protein of RLM (B) were incubated in KME + HEPES buffer at pH 7.50 and 37°C. The solid lines represent the simultaneous global fitting of all experimental points to the exclusive ligand binding MWC Equation 1. The χ2 values of the non-linear regression analyses were 0.00003 and 0.00013 for HepM GDH and RLM GDH, respectively. The Vmax values in ΔAbs/min at each ADP concentration were 0.037 ± 0.009 (0.13 mM ADP), 0.099 ± 0.010 (0.26 mM ADP), 0.153 ± 0.008 (0.5 mM ADP), and 0.193 ± 0.011 (1.5 mM ADP) for HepM GDH; and 0.131 ± 0.113 (0.25 mM ADP), 0.295 ± 0.022 (0.75 mM ADP), and 0.367 ± 0.024 (2.1 mM ADP) for RLM GDH.