Figure 8.

Simulation of GDH activity in the presence of modulators. For estimation of the GDH activity under physiological conditions, Equation 2 was used. The kinetic parameters at pH 7.5 were taken from Tables 2, 3, and concentrations of substrates and modulators were taken from Table 4. The gray-shaded area represents the range of ${\text{NH}}_4^{+}$ concentrations that can be found in cancer mitochondria when glutamine is oxidized (Table 4). In silico analysis was performed in the OriginPro 8 software with the following concentration values: 2.1 mM 2-oxoglutarate, 0.6 or 2 mM ADP, 0.14 mM GTP, and 1.2 mM NADPH.