. 2020 Apr 9;10:429. doi: 10.3389/fonc.2020.00429

Table 1.

Effect of ammonium supplementation on cancer cell proliferation.

	Duplication time (h)			μ (h^-1)			Maximal cellular densities (×10³)
	Control	NH₄Cl (mM)		Control	NH₄Cl (mM)		Control	NH₄Cl (mM)
		+5	+10		+5	+10		+5	+10
Metastatic cells
MDA-MB-231	32 ± 1	26 ± 3 ^*	25 ± 1^*	0.47 ± 0.05	0.61 ± 0.06^*	0.9 ± 0.07^*	82 ± 16	145 ± 13^*	142 + 17^*
HeLa	31 ± 3	25.5 ± 2^*	24 ± 2^*	0.63 ± 0.04	0.77 ± 0.03^*	0.8 ± 0.05^*	87 ± 9	153 ± 13^*	163 ± 9^*
PC3	36 ± 2	28 + 2^*	26 ± 1^*	0.57 ± 0.06	1.01 ± 0.04^*	1.05 ± 0.03^*	68 ± 3.5	136 ± 2^*	141 ± 1.4^*
HCT116	69 ± 3	45 ± 1^*	39 ± 2^*	0.36 ± 0.05	0.49 ± 0.03^*	0.51 ± 0.02^*	45 ± 7	76 ± 11^*	80 ± 13^*
Colo 205	80 ± 5	48 ± 2^*	44 ± 4^*	0.53 ± 0.02	0.62 ± 0.05^*	0.63 ± 0.04^*	45 ± 7	68 ± 6^*	66 ± 6^*
Low metastatic cells
MCF-7	34 ± 1	37 ± 2	45 ± 6	0.34 ± 0.05	0.38 ± 0.02	0.69 ± 0.03^*	44 ± 7	72 ± 6^*	76 ± 5^*
SiHa	48 ± 3	51 ± 4	55 ± 5	0.60 ± 0.04	0.58 ± 0.05	0.55 ± 0.07	90 ± 6	86 ± 7	82 ± 5
DU145	26 ± 2	32 ± 2^*	35 ± 3^*	1.0 ± 0.06	0.47 ± 0.07^*	0.55 ± 0.04^*	106 ± 8	92 ± 6	85 ± 4^*
A549	25 ± 3	20 ± 2	17 ± 3^*	0.78 ± 0.05	0.82 ± 0.04	0.85 ± 0.06^*	41 ± 5	49 ± 4	56 ± 2^*
Non-cancer cells
3T3	26 ± 0.5	46 ± 2^*	60 ± 1^*	0.7 ± 0.02	0.43 ± 0.1^*	0.32 ± 0.003^*	131 ± 5	45 ± 3^*	36 ± 2^*
HFF-1	32 ± 7	38 ± 12	55 ± 2^*	0.75 ± 0.1	0.65 ± 0.08	0.4 ± 0.1^*	93 ± 5	60 ± 10^*	46 ± 3^*

The number of independent cell cultures assayed (n) was 3. Statistical analysis was performed using one-way ANOVA with Scheffé comparison test.

P ≤ 0.05 or 0.01 vs. control; the cell viability in all conditions was >90%. The maximal cellular densities were reached for HeLa and MDA-MB-231 cells at day 4; for PC3, 3T3, HFF-1, and DU145 cells at day 5; for Colo-205, MCF-7, A549, and SiHa cells at day 6; and for HCT 116 cells at day 7.