Table 4.

Kinetic parameters of the GDH inhibition by GTP derived from the MWC equation.

GDH kinetic parameters	pH 7.2		pH 7.5
	HepM	RLM	HepM	RLM
Vmax, mU/mg protein	163 ± 78* (3)	848 ± 119 (3)	178 ± 56 (3)	714 (2)
Km ${\text{NH}}_4^{+}$, mM	12.0 ± 2.6 (3)	8.5 ± 4.5 (3)	10.3 ± 2.6 (3)	5.6 ± 3.6 (3)
n	1.99 ± 0.65 (3)	2.17 ± 0.25 (3)	3.3 ± 1.3 (3)	1.6 ± 0.2 (3)
L'	8.8 ± 11 (3)	20 ± 28 (3)	31 ± 34 (3)	14 ± 20 (3)
Ki GTP, μM	28 ± 27 (3)	60 ± 10 (3)	64 ± 59 (3)	31.7 ± 7 (3)

^*P < 0.001 vs. RLM. The number of independent preparations assayed is indicated between parentheses.