Figure 4.

YAP Promotes Pluripotency Induction in Mixed Cultures

(A) Schema of lentiviral vectors used to generate a mixture of cells that express OSKM, YAP, both, or neither.

(B–H) Experimental scheme illustrating the heterogeneous cell types co-existing in culture (B). The expression status of GFP from EV- or YAP-expressing vectors was determined in the resulting colonies, shown in (C–H). (C) Representative OSKM-mCherry+ colony images after 18 days of reprogramming from MEFs co-transduced with EV, wtYAP, or caYAP, each expressing a GFP reporter. (D) Quantification of total OSKM-mCherry+ colonies in (C). (E) Quantification of the OSKM-mCherry+ colonies also positive for GFP in (C). (F) Representative iPSC colonies after 18 days of reprogramming immunostained for the endogenous Nanog protein. (G) Quantification of total Nanog+ iPSC colonies in (F). (H) Quantification of Nanog+ iPSC colonies also positive for GFP in (F). Data from three biological replicates are displayed in (D–E, G, and H), repeated over at least three independent experiments.