Figure 3.

hiPSC-Derived NF1-Mutant Neurons, NPCs, and Nf1-Mutant Mice Display Molecular Similarities and Differences

(A) Nf1-mutant (1149C > A, 2041C > T, 3431-32_dupGT, 5425C > T) genetically engineered mouse brain lysates exhibit increased RAS activity compared with wild-type littermate controls.

(B) GABA levels are increased in all NF1-mutant NPC-derived neurons relative to controls.

(C and D) Dopamine levels are differentially reduced in (C) NF1-mutant NPCs relative to controls and (D) Nf1-mutant genetically engineered mouse brain lysates compared with WT littermate controls.

Each dot represents an independently generated data point derived from separate experiments and the two different clones for each line are denoted as black versus gray dots. All data are represented as means ± SEM. One-way ANOVA with Tukey post-test.