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. 2020 Apr 15;40(4):BSR20191845. doi: 10.1042/BSR20191845

Figure 5. miR-96-5p silence inhibits tumor growth and metastasis in MGC-803 cells.

Figure 5

(A) The mRNA level of ZDHHC5 in MGC-803 cells transfected with si-NC, si-ZDHHC5 by qRT-PCR. (B) Cell viability in MGC-803 cells transfected with miR-96-5p inhibitor, miR-96-5p inhibitor + si-ZDHHC5, or their corresponding controls by MTT assay. (C) The rate of apoptotic cells in MGC-803 cells transfected with miR-96-5p inhibitor, miR-96-5p inhibitor + si-ZDHHC5, or their corresponding controls by flow cytometry analysis. (D,E) Cell migration and invasion in MGC-803 cells transfected with miR-96-5p inhibitor, miR-96-5p inhibitor + si-ZDHHC5, or their corresponding controls by Transwell assay. (F) The protein levels of COX-2, Bcl-2, Bax, pro-caspase-3, cleaved-caspase-3, pro-caspase-9, and cleaved-caspase-9 in MGC-803 cells transfected with miR-96-5p inhibitor, miR-96-5p inhibitor + si-ZDHHC5, or their corresponding controls by Western blotting. (*) denotes difference from control (P<0.01); (**) denotes (P<0.05); (***) denotes (P>0.05). Values are means ± SD.