Figure 1.

Identification of a Conserved SEP Expressed by Adipocytes

(A) Alignment of LINC00116's conserved smORF and its homologs from 18 placental mammals, including translation of the SEP.

(B) A total of 483 expressed sequence tags (ESTs) from 33 distinct vertebrate species map over the human MTLN smORF. The distribution of the number of ESTs by species is shown. Xeno-ESTs are derived from translated BLAT alignments of ESTs in GenBank from non-human vertebrates (UCSC “xenoEst” track).

(C) Protocol for differentiation of hPSC-adipocytes. Adipogenesis begins with MPCs on day 21 of the protocol.

(D) qRT-PCR analysis of LINC00116 mRNA levels throughout adipogenesis. n = 3 independent biological replicate experiments.

(E) Primary sequence of the MTLN peptide. Peptides identified by mass spectrometry analysis of hPSC-adipocytes with their respective molecular weights in yellow.

(F) Immunoblot of whole-cell lysate from MTLN-FLAG knockin hPSC-adipocytes and lentivirus-delivered MTLN-FLAG-overexpressing hPSC-adipocytes. β-Actin was used as the loading control.

Data are means ± SD. Related to Figure S1.