Figure 3.

MTLN Localizes to the Mitochondrion and Affects Energetics Regulation

(A) Confocal microscopy showing MTLN-HA (red), ATP5B (green), neutral lipids (purple), and nuclei (blue) in differentiated hPSC-adipocytes. Scale bar, 20 μm.

(B) Immunogold labeling of MTLN-HA in differentiated hPSC-adipocytes.

(C) Subcellular fractionation of differentiated hPSC-adipocytes. Whole-cell lysate is blotted as input material.

(D) Basal and maximal fatty acid oxidation of hPSC-adipocytes. n = 35.

(E) Comparison of glycolysis, glycolytic capacity, and reserve in hPSC-adipocytes. n = 30. Bars represent mean values ±SD.

^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001, ^∗∗∗∗p ≤ 0.0001; two-way ANOVA. All n values are independent biological replicates. Related to Figure S3.