Figure 5.

Deletion of mTor, but Not Pparg, from C-KIT⁺ Cells Impaired Bone Development

All data (mean ± SD) reflected analyses of femurs from 2-month-old mice that had been tamoxifen treated at E12.5/14.5. Different sexes were examined in different experiments such that most data reflected both male and female mice. Two-tailed Student's t tests were used to assess the statistical significance of differences between sex-matched littermates (^∗∗p < 0.01).

(A and B) Representative microCT images showed trabecular (A) and cortical bones (B) from mTor^fl/fl and Kit^MerCreMer; mTor^fl/fl mice (n = 5 mice per genotype from three independent experiments).

(C–I) MicroCT analyses of the trabecular bone volume/total bone volume (C), trabecular number (D), spacing (E), thickness (F), cortical area (G), cortical area/total area (H), and thickness (I) of femurs from mTor^fl/fl and Kit^MerCreMer; mTor^fl/fl mice (n = 5 mice per genotype from three independent experiments).

(J) Frequencies of CD45⁻TER119⁻CD31⁻PDGFRα⁺ bone marrow stromal cells in Pparg^fl/fl and Kit^MerCreMer; Pparg^fl/fl mice (n = 5 mice from three independent experiments).

(K and L) Representative microCT images showed trabecular bone (K) and cortical bone (L) from Pparg^fl/fl and Kit^MerCreMer; Pparg^fl/fl mice (n = 5 mice per genotype from three independent experiments).

(M–S) MicroCT analyses of the trabecular bone volume/total bone volume (M), trabecular number (N), spacing (O), thickness (P), cortical area (Q), cortical area/total area (R), and thickness (S) of femurs from Pparg^fl/fl and Kit^MerCreMer; Pparg^fl/fl mice (n = 5 mice per genotype from three independent experiments).

(T) Frequencies of CD45⁻TER119⁻CD31⁻PDGFRα⁺ bone marrow stromal cells in Pparg^fl/fl and Kit^MerCreMer; Pparg^fl/fl mice (n = 5 mice per genotype from three independent experiments).