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. 2020 Mar 26;43(6):1964–1974. doi: 10.3892/or.2020.7565

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — USP19 overexpression inhibits clear cell renal cell carcinoma cell proliferation in vitro. Expression of USP19 was evaluated by (A) RT-qPCR and (B) western blotting in USP19-overexpressing or control Caki-1 cells. (C) Cell Counting Kit-8 assays and (D) and soft agar assays were performed to detect cell proliferation in USP19-overexpressing or control Caki-1 cells. (E) RT-qPCR analysis of PCNA, cyclin D1 and p27 mRNA levels in USP19-overexpressing or control Caki-1 cells. (F) Western blot analysis of PCNA, cyclin D1 and p27 protein levels in USP19-overexpressing or control Caki-1 cells. β-actin was used as the loading control, and 3 replicates of the indicated proteins were quantified by Image Lab software. Data are presented as the mean ± SD of three independent experiments. *P<0.05, **P<0.01 vs. ctrl. USP, ubiquitin specific peptidase; RT-qPCR, reverse transcription-quantitative PCR; PCNA, proliferating cell nuclear antigen; OD, optical density.