Figure 1.

Behavioral Abnormalities of Setd5^+/– Mice

(A) Social communication of neonatal Setd5^+/+ or Setd5^+/– mice assessed by the ultrasonic vocalization test at postnatal day 6. Call number over 5 min was determined. Data are means ± SEM (n = 30, Setd5^+/+; n = 25, Setd5^+/–). ∗p < 0.05 (rank-sum test).

(B) Social interaction of adult mice assessed in a novel environment. Two mice of the same genotype and sex that had been housed in different cages were placed together in a box. Mean duration time per contact and total duration time of contacts were determined. Data are means ± SEM (n = 8 for each genotype). ∗p < 0.05 by one-way analysis of variance (ANOVA).

(C) Association memory formation in adult mice assessed by the cued fear-conditioning test. A conditioned stimulus of sound (55-dB white noise for 30 s) and aversive unconditioned stimulus of foot shock (0.3 mA for 2 s) were given during the indicated periods. The percentage time for freezing behavior in consecutive blocks of 1 min during the conditioning phase (left panel) and cued testing on the following day (right panel) was determined. Data are means ± SEM (n = 17 for each genotype). ∗p < 0.05, ∗∗p < 0.01 (two-way repeated-measures ANOVA).

(D) Association memory retention in adult mice assessed by the cued fear-conditioning test. Cued testing as in (C) was performed 1 month after the last conditioning. Data are means ± SEM (n = 17 for each genotype). ∗p < 0.05, ∗∗p < 0.01 (two-way repeated-measures ANOVA).

(E) Activity level of adult mice assessed by monitoring of the home cage over 24 h. Two mice of the same genotype and sex that had been housed in different cages were placed together in a box, and their activity was continuously monitored over 24 h. Data for 7 days are shown compressed and are means ± SEM (n = 8 per genotype). ∗p < 0.05, ∗∗p < 0.01 (two-way repeated-measures ANOVA).

See also Figures S1–S4 as well as Table S1.