Table 1.
Antibody | Source (vendor/catalog number) | Dilution | Use |
---|---|---|---|
FABP-1 | Cell Signaling (Danvers, MA)/13368 | 1:200 | IF |
FABP-1 | Cell Signaling/13368 | 1:1000 | WB |
Cleaved caspase-3 | Cell Signaling/9664 | 1:1000 | WB |
Cleaved PARP | Cell Signaling/94885 | 1:1000 | WB |
Chromogranin A | Santa Cruz Biotechnology (Dallas, TX)/sc-393941 | 1:100 | IF |
Claudin 1 | Zymed (Waltham, MA)/71-7800 | 1:500 | WB |
Claudin 4 | Invitrogen (Waltham, MA)/364800 | 1:1000 | WB |
Claudin 7 | IBL (Gunma, Japan) Japan/18875 | 1:1000 | WB |
Claudin 7 | IBL Japan/18875 | 1:200 | IF |
Claudin 15 | IBL Japan/18805 | 1:500 | WB |
E-cadherin | BD Transduction Labs (San Jose, CA)/610181 | 1:1000 | WB |
Dcamkl1 | Abcam (Cambridge, MA)/ab37994 | 1:100 | IF |
GAPDH | Cell Signaling/2118 | 1:1000 | WB |
Hopx | Santa Cruz Biotechnology/sc-30216 | 1:500 | WB |
Lysozyme | Abcam/ab36362 | 1:200 | IF/IHC |
Muc2 | Santa Cruz Biotechnology/sc-7314 | 1:200 | IHC |
Nonphospho(active)β-catenin | Cell Signaling/8814 | 1:1000 | WB |
Olfm4 | Novus Biologicals (Centennial, CO)/NBP2-24535 | 1:800 | WB |
Olfm4 | Cell Signaling/39141 | 1:200 | IF |
Occudin | Zymed/71-1500 | 1:2000 | WB |
PCNA | Cell Signaling/2586 | 1:200 | IF |
Phopho-GSK3β(S9) | Cell Signaling/9336 | 1:1000 | WB |
Phospho–β-catenin (S33/37/T41) | Cell Signaling/9561 | 1:1000 | WB |
Sox9 | Millipore (Bedford, MA)/AB5535 | 1:100 | IF |
Tcf4 | Santa Cruz Biotechnology/sc-166699 | 1:1000 | WB |
Total GSK3β | BD Transduction Labs/610202 | 1:1000 | WB |
Total β-catenin | Cell Signaling/8480 | 1:1000 | WB |
Wnt3a | Santa Cruz Biotechnology/sc-74537 | 1:1000 | WB |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IF, immunofluorescence; IHC, immunohistochemistry; Muc2, mucin 2; S33/37/T41 serines 33 and 37, and threonine 41; WB, Western blot.