Figure 3.

Deficiency of caspase-1 improved liver function tests but increased liver fibrosis after 7 days of BDL in mice (WT sham, n = 7; WT BDL, n = 7; Casp1^–/–sham, n = 5; Casp1^–/–BDL, n = 6). (A) Plasma levels of ALT, ALP, IL-1β, and bile acid, and bile acid levels in livers. (B) Hematoxylin and eosin histology assessment (scores) of liver necrosis, fibrosis, inflammation, and bile duct proliferation. (C) Biochemical analysis of liver hydroxyproline content and Western blot analysis of smooth muscle actin (SMA) protein expression. GAPDH was used as loading control and data normalization, data presented as mean ± SD.