Table 5.
List of Fluorochrome-Conjugated Primary Antibodies
| Antibody | Isotype | Company | Clone | Fluorochromes |
|---|---|---|---|---|
| CD4 | Mouse IgG1, κ | BD Pharmingen | RPA-T4 | APC |
| CD4 | Mouse IgG1, κ | Miltenyi | M-T466 | FITC |
| CD8 | Human IgG1 | Miltenyi | REA734 | APC |
| CD8 | Mouse IgG1, κ | BD Pharmingem | HIT8a | PE |
| CD8 | Mouse IgG1, κ | Biolegend | HIT8a | BV510 |
| CD3 | Mouse IgG2a, κ | Biolegend | OKT3 | BV421 |
| CD14 | Human IgG1 | Miltenyi | REA599 | PE |
| CD14 | Mouse IgG1, κ | Biolegend | HCD14 | BV421 |
| CD49d (beta 7 integrin) | Mouse IgG1, κ | BD Pharmingen | 9F10 | APC |
| CD69 | Mouse IgG1, κ | BD Pharmingen | N50 | APC |
| CD103 (alpha E integrin) | Mouse IgG1, κ | Biolegend | Ber-ACT8 | APC |
| CD161 | Mouse IgG1, κ | Biolegend | HP-3G10 | PE |
| CD161 | Mouse IgG1, κ | Biolegend | HP-3G10 | APC |
| CD183 (CXCR3) | Mouse IgG1, κ | BD Pharmingen | 1C6/CXCR3 | APC |
| CD195 (CCR5) | Mouse IgG2a | BD Pharmingen | 2D7/CCR5 | APC |
| CD196 (CCR6) | Mouse IgG1, κ | BD Pharmingen | 11A9 | APC |
| CD197 (CCR7) | Mouse IgG2a | Biolegend | G043H7 | APC |
| CD279 (PD-1) | Mouse IgG1, κ | Biolegend | EH12.2H7 | APC |
| GATA-3 | Human IgG1 | Miltenyi | REA174 | APC |
| Granzyme B | Mouse IgG1, κ | Biolegend | QA16A02 | APC |
| IFN-γ | Mouse IgG1, κ | Biolegend | 4S.B3 | APC |
| IL-17A | Mouse IgG1, κ | Biolegend | BL168 | APC |
| Ki-67 | Human IgG1 | Miltenyi | REA183 | APC |
| TNF alpha | Mouse IgG1, κ | Biolegend | MAb11 | APC |
| MR1 | Mouse IgG2a, κ | Biolegend | 26.5 | APC |
| MR1/6-FP | Tetramer | NIH tetramer core facility | APC/FITC | |
| MR1/5-OP-RU | Tetramer | NIH tetramer core facility | APC/FITC | |
| Perforin | Mouse IgG1 | Biolegend | B-D48 | APC |
| RORγ(t) | Human IgG1 | Miltenyi | REA278 | APC |
| T-bet | Human IgG1 | Miltenyi | REA102 | APC |
| TCR Vα7.2 | Mouse IgG1, κ | Biolegend | 3C10 | FITC |
| TCR Vα7.2 | Mouse IgG1, κ | Biolegend | 3C10 | APC |