Skip to main content
. 2020 Apr 9;11:266. doi: 10.3389/fphys.2020.00266

FIGURE 6.

FIGURE 6

Adjuvant effect of CS and small molecule inhibitors on histopathology. In the CHOP siRNA group (A–D), the controls were unventilated (A), and showed interstitial thickening between the pneumocytes with hyaline membrane deposits in the alveolar spaces. Although CS treatment helped in the recovery of lung injury in the CHOP siRNA group (B), treatment with CHOP siRNA alone (C) did not show any significant improvement, but there was significant improvement in the CHOP siRNA + CS group (D) as shown in the Lung Injury Scoring Index graph. In the Ang2 siRNA group (E–H), the controls were unventilated (E), and showed interstitial thickening between the pneumocytes with hyaline membrane deposits in the alveolar spaces. CS treatment did not help in the recovery of lung injury in the (F), treatment with Ang2 siRNA alone (G) or in combination with CS (H) showed significant improvement, as shown in the Lung Injury Scoring Index graph. In the miR34a Inh group (I–M), the controls were unventilated (I), or ventilated (J) with the latter improvement. CS treatment alone significantly helped in the recovery of lung injury (K), which was also noted in the animals treated with miR34a Inh alone (L) or in combination with CS (M), as shown in the Lung Injury Scoring Index graph. Arrows represent hyaline membrane deposits. Scale bar: 200μm; ∗p ≤ 0.05; ∗∗p ≤ 0.001; ∗∗∗p ≤ 0.0001; (N = 6–8). CS: Curosurf® (surfactant); CHOP: CCAAT enhancer-binding protein, i.e., C/EBP homologous protein, also known as growth arrest and DNA damage-inducible gene 153/GADD153 or Ddit3; si: silencing; Ang2: Angiopoietin 2; miR: microRNA; Inh: inhibitor.