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. Author manuscript; available in PMC: 2020 Apr 16.
Published in final edited form as: Cell Rep. 2018 Feb 27;22(9):2431–2441. doi: 10.1016/j.celrep.2018.02.027

Figure 3. Mitochondria Significantly Contribute to the Killing of Δhla S. aureus.

Figure 3.

(A) Percentage of WT or Δhla S. aureus killed following 1-hr incubation with mitoTEMPO- or DMSO-treated (30 min) primary human monocytes.

(B) Confocal images of mitochondria (MitoTracker) and WT or Δhla S. aureus within live primary human monocytes. Scale bar, 5 μm; yellow box indicates the magnified area.

(C) Quantification of the percentage of internalized WT or Δhla S. aureus associated with a mitochondria. n > 100 combined from 3 individual experiments.

(D) Confocal images of mitochondria (MitoTracker) and WT S. aureus within live primary human monocytes treated with MCC950 or DMSO. Scale bar, 5 μm; yellow box indicates the magnified area.

(E) Quantification of the percentage of internalized S. aureus associated with a mitochondria in cells treated with MCC950 or DMSO. n > 50 combined from 3 individual experiments.

Statistical significance (A, C, and E) was determined by Mann-Whitney test. All data are representative of 3 independent experiments. (A) n ≥ 4 replicates per group. Data presented as mean ± SD.