Figure 4. ETC Complex II Activity Contributes to the Killing of Δhla S. aureus.

(A) TMRM analysis of the mitochondrial membrane potential in naive primary human monocytes, or monocytes incubated (MOI 1) with WT or Δhla S. aureus.

(B) Seahorse analysis of mitochondrial respiration in naive primary human monocytes, or monocytes incubated (MOI 1) with WT or Δhla S. aureus.

(C) Percentage of S. aureus killed following 1-hr incubation with primary human monocytes treated (2 hr) with rotenone (ROT), DMM, CCCP, or DMSO.

(D) Percentage of Δhla S. aureus killed following 1-hr incubation with primary human monocytes treated (2 hr) with rotenone (ROT), DMM, CCCP, or DMSO.

(E) Percentage of S. aureus killed following 1-hr incubation with primary human monocytes treated (2 hr) with DMM, iNLRP3, or both DMM and iNLRP3.

(F) Numbers of S. aureus CFUs recovered following 3-hr incubation in PBS containing DMSO, diethyl succinate, or dimethyl fumarate.

(G) Numbers of S. aureus CFUs recovered from the peritoneum and kidneys of mice infected (i.p., 1e6 CFUs) with WT or Δhla S. aureus along with 600 mg/kg DMM.

(H) Numbers of S. aureus CFUs recovered from the lungs of mice treated with 600 mg/kg DMM or DMSO (i.p., time of infection) and infected (IN, 1e6 CFUs) with WT or Δhla S. aureus. Statistical significance (C–E and H) was determined by ANOVA followed by Dunn’s test or

(G) Mann-Whitney test. All data are representative of at least 2 independent experiments. (A–F) n ≥ 4 replicates per group. Data presented as mean ± SD.